• Beauty & Skincare
    • Beauty Supply
    • Nails How-Tos and Tips
    • Natural Beauty 101
    • Quick Beauty Tips
    • Skin Care Advice & Products
  • Makeup
  • Hairstyle Tutorials
    • Women’s Hairstyles
    • Men’s Hair
  • Fashion & Style
    • Baby Clothes
    • Budget Style
    • Celebrity Style
    • DIY Fashion
    • Jeans and Denim
    • Men’s Fashion
    • Lingerie – A Style Guide
    • Personal Style
    • Plus-Size Fashion
  • Tattoos & Piercings
  • Lifestyle
    • Career Management Tips, Advice
    • Cultural Feature Stories
    • Education
    • Food & Drink
    • Health and Wellness
    • Homes and Interiors
    • Travel
    • Dating and Relationships
  • Shoes, Bags
  • Accessories
  • Jewelry
    • Diamonds Guide
    • Engagement Rings
    • Gemstones Buying Advice
    • Jewelry Advice & How To’s – Jewelry Tips
    • Jewelry Trends & News
  • More
    • Special Occasions
    • Fashion Blog
  • Contact
  • About
What's Hot

Wedding Preparation – guest list children invitation and wedding style

February 3rd, 2023

Your dream wedding preparation starts from here

February 3rd, 2023

5 Self-Care Weekend Ideas to Unwind & Relax

February 3rd, 2023
Facebook Instagram Pinterest
Blufashion – Best Beauty BlogBlufashion – Best Beauty Blog
  • CONTACT
  • ABOUT
SUBSCRIBE
  • Beauty, Skincare & Hairstyles
    • Women’s Hairstyles
    • Men’s Hair
      • Care & Styling
      • Product Reviews
    • Hairstyle Tutorials
    • Skin Care Advice & Products
    • Natural Beauty
    • Beauty Tips
    • Beauty Supply
    • Makeup Tips & Tutorials
      • Body Basics
        • Face Fundamentals
    • Nails
    • Tattoos & Body Piercings
  • Fashion
    • Women’s Fashion
    • Men’s Fashion
    • Personal Style
    • Shopping Tips
    • Budget Style
    • Celebrity Style
    • DIY Fashion
    • Jeans & Denim
    • Plus-Size Fashion
    • Swimwear & Bikinis
    • Lingerie
    • Baby Clothes
  • Lifestyle
    • Food & Drink
    • Health & Wellness
    • Homes and Interiors
    • Travel
    • Career
    • Culture
    • Education
    • Finance
    • Tech
  • Shoes, Bags & Jewelry
    • Bags, Handbags and Purses
    • Jewelry
      • Engagement Rings
      • Antique Jewelry
      • Jewelry Tips
      • Jewelry Trends
      • Body Jewelry
      • Diamonds Guide
      • Gemstones Buying Advice
      • Earring – Shop for Earrings
      • Pearls
    • Accessories
    • Watches
    • Shoes
      • Women’s Shoes
      • Men’s Shoes
      • Kids & Baby Shoes
      • Shoe Styles
      • Online Shoes Stores
      • Shoe Repair, Care & Storage
  • More
    • Special Occasions
    • Tattoo
    • Eyeglasses and Sunglasses
    • Fashion Blog
    • Pet Care
    • Parenting
    • Dating and Relationships
Blufashion – Best Beauty BlogBlufashion – Best Beauty Blog
Home»Lifestyle»Health and Wellness»3 Important human enzymes: MPO, PTEN and ADA
Health and Wellness

3 Important human enzymes: MPO, PTEN and ADA

Iskra BanovićBy Iskra BanovićFebruary 11th, 2022Updated:September 5th, 2022No Comments4 Mins Read
Facebook Twitter Pinterest LinkedIn Tumblr WhatsApp Telegram Email
Share
Facebook Twitter LinkedIn Pinterest Email

Enzymes are important proteins in the human body. They aid in the acceleration of chemical reactions in the human body. They attach themselves to molecules and change them in precise ways. They are required for breathing, digestion, muscle and nerve function, and a variety of other functions. In this article, We are going to look deeper into 3 important enzymes , MPO, PTEN, and ADA.

3 Important human enzymes: MPO, PTEN and ADA
(Image Credits: Genome.gov)

What is Myeloperoxidase(MPO)? 

MPO (myeloperoxidase) is a peroxidase enzyme encoded by the MPO gene on chromosome 17 in humans. MPO is most abundantly expressed in neutrophil granulocytes (a subtype of white blood cells) and creates hypohalous acids, particularly hypochlorous acid (the sodium salt of which is the ingredient in bleach), to carry out their antibacterial action.

During the neutrophil’s respiratory burst, MPO, a member of the XPO subfamily of peroxidases, creates hypochlorous acid (HOCl) from hydrogen peroxide (H2O2) and chloride anion (Cl) (or hypobromous acid if Br- is present). Heme is required as a cofactor. It also uses hydrogen peroxide as an oxidizing agent to convert tyrosine to tyrosyl radical. Because hypochlorous acid and the tyrosyl radical are both cytotoxic, the neutrophil uses them to kill bacteria and other pathogens.

Clinical Significance of MPO antibody

MPO antibody, when used in tandem with other autoantibody tests, can be used to assess patients with immune-mediated vasculitis, particularly microscopic polyangiitis (MPA). Patients with MPA are more likely to have MPO ANCA, and azotemia related to glomerulonephritis is a possibility (pauci-immune necrotizing glomerulonephritis). MPO ANCA is present in patients with systemic lupus erythematosus with or without lupus nephritis, Goodpasture syndrome, and Churg-Strauss syndrome, as well as people with MPA. In lupus nephritis, Goodpasture disease, and Wegener granulomatosis, azotemia with progressive renal failure can occur. Autoantibody testing may be helpful in distinguishing certain illnesses if clinical signs and symptoms are lacking.

How to make the best out of MPO antibody

Humans, mice, and rats are all reactive to MPO antibodies. The antibody has been tested in immunohistochemistry, immunofluorescence, and Western blotting. 2g/ml for immunofluorescence, 0.5-1g/ml for paraffin-embedded immunohistochemistry, and 0.1-0.5g/ml for Western blotting are recommended test dilutions. When it comes to assay optimization, the guidelines above are an excellent place to start. Working concentration varies, and the user has the final say.

What is PTEN? 

Phosphatase and tensin homolog (PTEN) is a phosphatase that the PTEN gene encodes in humans. Through the activity of its phosphatase protein product, PTEN operates as a tumor suppressor gene. This phosphatase plays a role in cell cycle regulation, stopping cells from expanding and dividing too quickly. Many anticancer medications target it. The PTEN enzyme binds to another PTEN enzyme (dimerizes) to function, then binds to the cell membrane. By eliminating phosphate groups, which are made up of a cluster of oxygen and phosphorus atoms, the PTEN enzyme alters other proteins and fats (lipids). Phosphatases are enzymes that perform this function.

About PTEN antibody 

PTEN Antibody detects total PTEN protein in the body. The antibody has no cross-reactivity with proteins that are linked to it. PTEN Antibody is a high-quality monoclonal PTEN antibody (also known as phosphatase and tensin homolog antibody) that can be used to detect mouse, rat, and human PTEN proteins. PTEN Antibody is available as a non-conjugated anti-PTEN antibody as well as a variety of conjugated anti-PTEN antibody options.

What is ADA? 

The ADA gene gives instructions for making the adenosine deaminase enzyme. Adenosine deaminase is produced by all cells, although it is found in the highest concentrations in immune system cells termed lymphocytes, which grow in lymphoid organs. The thymus gland, which is positioned behind the breastbone, and lymph nodes, which are spread throughout the body, are examples of lymphoid tissues.

Adenosine deaminase (ADA) is a purine metabolic enzyme that plays a key role in lymphoid cell growth and function. This enzyme belongs to the hydrolase family, which includes enzymes that operate on carbon-nitrogen bonds other than peptide bonds, such as cyclic amidines. Adenine aminohydrolase is the scientific name for this enzyme class.

About  ADA antibody 

The ADA antibody detects total Adenosine Deaminase protein in the body. The recombinant fragment has a sequence that corresponds to a portion of Adenosine Deaminase between amino acids 119 and 363. Antigen-affinity chromatography is used to purify ADA antibodies.

Share. Facebook Twitter Pinterest LinkedIn Tumblr Email
Iskra Banović
Iskra Banović
  • Website
  • Facebook
  • Pinterest
  • Instagram

Hello out there to all you fashion-hungry Blufashion.com readers! My name is Iskra Banovic, and I'm a fashion writer. As the fashion expert for www.blufashion.com, I wish to bring a creative, informative, and inspiring aspect to the fashion content you have come to trust.

Read more related stories:

How to Start Running for Weight Loss

Celtic Sea Salt & Its Health Benefits

Everything You Need To Know About Abu Dhabi’s Health Authority

How to Legally Deal with the Consequences of Cerebral Palsy

How To Choose Your Cosmetic Surgeon?

How Eating Healthy Foods Can Help You Prevent Hair Loss and Maintain Healthy Hair?

Add A Comment

Leave A Reply Cancel Reply

Latest Articles

Wedding Preparation – guest list children invitation and wedding style

February 3rd, 2023

Your dream wedding preparation starts from here

February 3rd, 2023

5 Self-Care Weekend Ideas to Unwind & Relax

February 3rd, 2023

Men Shopping for Women’s Shoes

February 2nd, 2023
  • Facebook
  • Pinterest
  • Instagram
  • YouTube
Don't Miss

How to Make The Mother of all DIY Face Peels

Today, we have for you a guide to making the big-daddy of home-made face-peels. And…

5 Tips to Increase Jewelry Store Sales

October 23rd, 2022

Is Lancome Artliner Really Worth The $30 Price Tag?

October 23rd, 2022

LoweLew Review – High Quality Designer Replica Handbags

April 18th, 2020
Pinterest Instagram YouTube LinkedIn Facebook
  • About
  • Contact
  • Press
  • Team
  • Disclaimer
  • TOS
  • Privacy Policy
  • Ethics Policy
  • Affiliate Disclosure
© 2023 Blufashion - Best Beauty Blog.

Type above and press Enter to search. Press Esc to cancel.